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WB detection
release time：
2022-07-12 10:35
Principle: Polyacrylamide gel electrophoresis is used, the detected object is protein, the "probe" is an antibody, and the "color development" is a labeled secondary antibody. The protein samples separated by PAGE (polyacrylamide gel electrophoresis) are transferred to a solid phase carrier (such as PVDF membrane), which adsorbs proteins in a non-covalent form and can maintain the types of polypeptides and their biological properties separated by electrophoresis. The academic activity remains unchanged. The protein or polypeptide on the solid-phase carrier is used as the antigen, and it reacts with the corresponding antibody, and then reacts with the enzyme or isotope-labeled secondary antibody, and the specific purpose of electrophoretic separation is detected by substrate color development or autoradiography. The protein component of gene expression. This technique is also widely used to detect expression at the protein level,Then react with the enzyme or isotope-labeled secondary antibody, and detect the protein components expressed by the specific target gene separated by electrophoresis through substrate color development or autoradiography. This technique is also widely used to detect expression at the protein level.
Ruicheng WB testing service advantages:
1.Ensure the accuracy of the experiment, strict operation and quality control.
2.Demonstration explanations can be given for each experimental result.
3. Comprehensive technical services and complete technical service reports.
Service Process:
Obtain protein samples to be tested→protein assay→gel electrophoresis→Transfer film exposure→Development analysis
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