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Quantitative PCR detection
release time：
2022-07-12 10:39
Quantitative PCR: PCR, the abbreviation of polymerase chain reaction, is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication in vitro. Substantially increase the trace amount of DNA.
Conventional PCR technology is to quantitatively and qualitatively analyze the final product of PCR amplification reaction, while quantitative PCR, also known as real-time PCR or real-time quantitative PCR, refers to the use of fluorescent dyes or fluorescently labeled specific probes in the exponential amplification of PCR. During the amplification period, the quantitative and qualitative analysis of the starting template is realized by real-time detection of the fluorescent signal of each cycle product in the PCR amplification reaction.The technology has strong specificity, high sensitivity, few contamination routes, and accurate detection methods. It has achieved a leap from qualitative to quantitative PCR, and has become an internationally recognized standard method for nucleic acid quantification. It is widely used in gene expression abundance, copy number, etc. Research and clinical disease detection and other fields.
The company's quantitative PCR technology services use SYBR Green I fluorescent dye method or TaMman fluorescent probe method to perform loudness quantification or absolute quantification of DNA/RNA. Relative quantification: Calculate and compare the difference in the expression endorsement of the internal reference gene and the target gene by the 2-△△Ct method for relative quantification;
Absolute quantification: first prepare the standard, then detect and make a standard curve while detecting the target gene, and perform absolute quantification of the sample of unknown concentration by comparing the standard curve, and finally calculate the copy number, mole number or number of DNA/RNA to be tested in the sample. micrograms, etc.
The customer only needs to provide the experimental purpose, the information of the target gene to be tested, and the experimental sample, and the company can deliver the accurate and true original data and test report, so that the customer has no worries.
Ruicheng quantitative PCR service advantages:
1.There are many service methods: customers can choose SYBR Green1 fluorescent dye method or TaqMan fluorescent probe method according to their own experimental needs
2.High accuracy: use specific probes to identify quantitative molecules.
3.High efficiency: fast speed, high throughput, can complete the quantitative analysis of 96 samples in 2-3 hours.
Service Process:
RNA extraction→RNA concentration assay→Primer design→RNA reverse transcription→PCR pre-experiment→Quantitative PCR→Data analysis and reporting
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