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Elisa Test

release time:

2022-07-12 10:53


ELISA is an enzyme-linked adsorption reaction, which is an immunoenzyme technology developed after immunofluorescence and radioimmunoassay.
Principle: The basis of ELISA is the immobilization of antigen or antibody and the enzymatic labeling of antigen or antibody. The antigen or antibody bound on the surface of the solid phase carrier still retains its immunological activity, and the enzyme-labeled antigen or antibody retains both its immunological activity and the enzymatic activity.
During detection, the test substance (antigen or antibody) in the sample binds to the immobilized antibody or antigen. The unbound substances are removed by washing the plate, and then the enzyme-labeled antigen or antibody is added. At this time, the amount of enzyme that can be immobilized is related to the amount of the tested substance in the sample. By adding the substrate that reacts with the enzyme, the color is developed, and the content of the substance in the sample can be judged according to the depth of the color, and the qualitative or quantitative analysis can be carried out.
Ruicheng ELISA testing service advantages:
1.Good technology: Ruicheng technical service personnel have rich operating experience and superb technology, and they are careful and strict, and can complete the inspection efficiently, quickly and accurately.
2.Low price: Ruicheng provides customers with high-quality services at the most affordable price and helps customers save costs.
3.Short cycle: Ruicheng has complete equipment and experimental platform, which can provide customers with satisfactory services in the shortest time.
Service Process:
Confirm sample information→Add dilution standard→Add sample to be tested→Add enzyme-labeled antigen or antibody→Add substrate to catalyze enzyme color development→Add stop solution→Measure absorbance→Result analysis