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Whole Blood Dna Extraction Kit (spin Column Method)
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category
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Product Details
|
Article |
NoName |
Specifications |
|
RC12311 |
Whole blood DNA extraction kit (column method) |
50 times/box |
|
RC12312 |
Whole blood DNA extraction kit (column method) |
100 times/box |
|
RC12313 |
Whole blood DNA extraction kit (column method) |
250 times/box |
product description:
Principle: It mainly relies on the filter membrane to adsorb nucleic acids, while proteins and salts are not adsorbed and thus removed by filtration.
Product Description:
This kit consists of proteinase K, proteinase K lysing solution, lysing solution, washing solution 1, washing solution 2, and eluent adsorption column set.
Product advantages:
This kit provides a fast and flexible method for the extraction of total DNA from fresh blood and frozen whole blood. This kit can process 0.2-18ml of whole blood. It can effectively remove protein, lipid and other inhibitory impurities by using spin column method without using chloroform. High extraction purity and good quality can meet the requirements of downstream experiments.
Applications:
This product is suitable for nucleic acid extraction, enrichment, purification and other steps; the processed product is used for clinical in vitro detection.
Product Manual:
1. In a 1.5 ml centrifuge tube, add 25 μl of proteinase K solution.
2. Add 10-250 μl of the sample to be extracted into the tube, shake and mix for 5 seconds. (If the total volume is less than 250μl after adding the sample, the eluent can be used to make up the volume to 250μl)
3. Add 250 μl of lysis buffer to the sample. Mix by inversion 3-5 times and vortex at high speed for 15 seconds. 70°C water bath for 10 minutes.
4. Add 250 μl absolute ethanol to the sample and vortex at high speed for 10 seconds.
5. Load the gDNA column in a new collection tube. Transfer the mixture to the column. Centrifuge at 10,000 rpm for 1 minute and discard the waste liquid.
6. Add 500 μl of Wash 1 to the column. Centrifuge at 10,000 rpm for 1 minute and discard the waste liquid.
7. Add 600 μl of Wash 2 to the column. Centrifuge at 10,000 rpm for 1 minute and discard the waste liquid.
8. Put the column back into the collection tube and centrifuge at 10,000 rpm for 2 minutes.
9. Transfer the column to a new 1.5ml centrifuge tube and add 50μl of eluent pre-warmed to 70°C to the center of the membrane. Leave at room temperature for 2 minutes. Centrifuge at 10,000 rpm for 1 minute.
10. Discard the DNA collection column and store the obtained DNA at 2-8°C, and it is recommended to store it at -20°C for long-term storage.
Key words:
Purified Inter Hole Difference
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