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Whole Blood Dna Extraction Kit (magnetic Bead Method)
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category
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Product Details
|
Article |
NoName |
Specifications |
|
RC12421 |
Whole blood DNA extraction kit (magnetic bead method) |
48 times/box |
|
RC12422 |
Whole blood DNA extraction kit (magnetic bead method) |
96 times/box |
|
RC12423 |
Whole blood DNA extraction kit (magnetic bead method) |
250 times/box |
product description:
Principle: This method uses nano-scale magnetic beads. The surface of the magnetic beads is marked with a functional group, which can react with nucleic acid by adsorption.
Product Description:
This kit consists of proteinase K, proteinase K lysing solution, lysing solution, washing solution 1, washing solution 2, eluent, and magnetic bead solution.
Product advantages:
The purification efficiency is high, and the high-quality extraction of nucleic acid can be completed in a short time. The specificity is high, and the proposed nucleic acid purification effect is good. Provides the best solution system with minimal manual handling. It can cooperate with nucleic acid extractor to achieve fully automatic and efficient extraction effect.
Applications:
This product is suitable for nucleic acid extraction, enrichment, purification and other steps; the processed product is used for clinical in vitro detection.
Product Manual:
1. In a 1.5ml centrifuge tube, add 20μl of proteinase K solution.
2. Add 10-250μl of the sample to be extracted to the tube, shake and mix for 5 seconds. (If the total volume is less than 250μl after adding the sample, the eluent can be used to make up the volume to 250μl)
3. Add 250 μl of Lysis Buffer to the sample. Mix by inversion 3-5 times and vortex at high speed for 15 seconds. 70°C water bath for 10 minutes.
4. Add 25μl of magnetic bead solution to the sample, invert and mix 5-10 times, and let stand for 5 minutes at room temperature.
5. Transfer to a magnetic stand for 5-10 minutes and discard the waste liquid.
6. Add 500 μl of Wash 1 to the tube. After thorough mixing, transfer to a magnetic stand for adsorption for 2 minutes, and discard the waste liquid.
7. Add 500 μl of Wash Buffer 2 to the tube. After thorough mixing, transfer to a magnetic stand for adsorption for 2 minutes, and discard the waste liquid.
8. Add 500 μl of 75% absolute ethanol, mix well, transfer to a magnetic stand for adsorption for 2 minutes, and discard the waste solution.
9. Repeat step 8 once.
10. After a brief centrifugation, transfer the tube to a magnetic stand, pipette off all the solution, open the lid and let it dry for 5 minutes.
11. Add 20-100 μl of Eluent EB. Incubate with shaking at 55°C for 10 minutes, and then transfer to a magnetic stand for adsorption for 5 minutes. Transfer the DNA solution to a new tube for storage.
Key words:
Purified Inter Hole Difference
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